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Recombinant Mesothelin 抗体 (Extracellular Domain)

Name: Recombinant Mesothelin 抗体 (Extracellular Domain)
Brand: antibodies-online
SKU: ABIN7543372
Availability: OutOfStock

This anti-Mesothelin antibody is a 兔 Chimeric antibody detecting Mesothelin in ELISA, WB, IHC, FACS, IP 和 SPR. Suitable for 人.

产品编号 ABIN7543372

发货至: 中国

Quick Overview for Recombinant Mesothelin 抗体 (Extracellular Domain) (ABIN7543372)

抗原

See all Mesothelin (MSLN) 抗体

Mesothelin (MSLN)

抗体类型

Recombinant Antibody

适用

人

宿主

兔

克隆类型

Chimeric

标记

This Mesothelin antibody is un-conjugated

应用范围

ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Surface Plasmon Resonance (SPR)

克隆位点

MN-1

Discover our top product MSLN Primary Antibody

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Extracellular Domain

原理

Anti-Mesothelin [MN-1], Rabbit IgG, kappa

特异性

This antibody can recognize and bind human mesothelin. This antibody binds a different antibody on the human mesothelin than another antibody called MB. Mesothelin (MSLN) is a glycophosphatidylinositol (GPI)-linked cell surface protein highly expressed in several types of malignant tumors sometimes in association with increased tumor aggressiveness and poor clinical outcome. The membrane-anchored forms of this molecule may play a role in cellular adhesion.

产品特性

Original Species of Ab: Mouse
Original Format of Ab: IgG2a

纯化方法

Protein A affinity purified

免疫原

The original antibody was generated in mesothelin-deficient mice by immunization with plasmid cDNA corresponding to the mesothelin extracellular domain followed by a single boost with a recombinant human mesothelin-rabbit IgG Fc fusion protein.

亚型

IgG kappa
anti-Mesothelin (MSLN) (AA 273-407) antibody
MSLN 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal MSLN-2131 unconjugated

anti-Mesothelin (MSLN) (AA 101-200) antibody
MSLN 适用: 人 ELISA, WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Mesothelin (MSLN) (AA 273-407) antibody
MSLN 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal unconjugated

anti-Mesothelin (MSLN) (AA 37-286) antibody
MSLN 适用: 人, 猴 ELISA, WB, FACS 宿主: 小鼠 Monoclonal 5C11A4 unconjugated

anti-Mesothelin (MSLN) (AA 37-598) antibody
MSLN 适用: 人 ELISA, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated

anti-Mesothelin (MSLN) (AA 296-580) antibody
MSLN 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM71 unconjugated Recombinant Antibody

anti-Mesothelin (MSLN) (AA 296-606) antibody
MSLN 适用: 人, 大鼠 ELISA, WB 宿主: 小鼠 Monoclonal 7E6A6 unconjugated

anti-Mesothelin (MSLN) (AA 37-286) antibody
MSLN 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4H12F8 unconjugated

anti-Mesothelin (MSLN) (AA 1-62) antibody
MSLN 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mesothelin (MSLN) antibody
MSLN 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated

应用备注

The binding characterization of this antibody was done using ELISA and SPR. The binding affinity of the original IgG2a antibody for mesothelin-Fc protein was determined by BIACore and reported to be Kd = 1.0 nM. This antibody can recognize membrane bound mesothelin on the surface of mesothelin positive H226, A1847, Hela and Panc3.014 cells (mesothelin-positive) in a flow cytometry. This antibody can also bind denatured human mesothelin in a western blot assay. This antibody was also used in the immunohistochemical staining of cancerous tissue like ovarian adenocarcinoma and normal tissues like peritoneal mesothelium on uterus, pleural mesothelium on lung and fallopian tube luminal fluid (PMID: 16115924). This antibody was also used in an immunohistochemical analysis of 1562 tumors for mesothelin expression. It was reported that mesothelin was highly expressed in ovarian serous carcinoma, pancreatic adenocarcinoma and malignant pleural mesothelioma (PMID: 28460459). This antibody was also used in the immunoprecipitation of mesothelin from cell lysates (PMID: 23694968).

说明

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

PBS with 0.02 % Proclin 300.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
抗原

Mesothelin (MSLN)

别名

Mesothelin

背景

MSLN, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, MN

UniProt

Q13421

途径

EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process